Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase

@article{Mennella2005FunctionallyDK,
  title={Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase},
  author={Vito Mennella and Gregory C Rogers and Stephen L. Rogers and Daniel W Buster and Ronald D. Vale and David J. Sharp},
  journal={Nature Cell Biology},
  year={2005},
  volume={7},
  pages={235-245}
}
Regulation of microtubule polymerization and depolymerization is required for proper cell development. Here, we report that two proteins of the Drosophila melanogaster kinesin-13 family, KLP10A and KLP59C, cooperate to drive microtubule depolymerization in interphase cells. Analyses of microtubule dynamics in S2 cells depleted of these proteins indicate that both proteins stimulate depolymerization, but alter distinct parameters of dynamic instability; KLP10A stimulates catastrophe (a switch… Expand
The Dynamic Duo of Microtubule Depolymerization
TLDR
Findings have revealed remarkable coordination between two family members, KLP10A and KLP59C, in which alterations in the relative targeting of these proteins allows them to participate in markedly different tasks at distinct points in the cell cycle. Expand
KLP10A and KLP59C: The Dynamic Duo of Microtubule Depolymerization
TLDR
Findings have revealed remarkable coordination between two family members, KLP10A and KLP59C, in which alterations in the relative targeting of these proteins allows them to participate in markedly different tasks at distinct points in the cell cycle. Expand
Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells.
TLDR
The dynamics of five green fluorescent protein (GFP)-tagged mitotic kinesins in live Drosophila Schneider cell line (S2) are visualized, revealing a diverse spectrum of regulatory mechanisms for controlling the localization and function of five mitoticKinesins at different stages of the cell cycle. Expand
Cell Cycle-dependent Dynamics and Regulation of Mitotic Kinesins in Drosophila S 2 Cells □
Constructing a mitotic spindle requires the coordinated actions of several kinesin motor proteins. Here, we have visualized the dynamics of five green fluorescent protein (GFP)-tagged mitoticExpand
The Drosophila kinesin-13, KLP59D, impacts Pacman- and Flux-based chromosome movement.
Chromosome movements are linked to the active depolymerization of spindle microtubule (MT) ends. Here we identify the kinesin-13 family member, KLP59D, as a novel and uniquely important regulator ofExpand
Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration
TLDR
The Drosophila katanin Dm-Kat60 functions to generate a dynamic cortical-microtubule interface in interphase cells and removes tubulin from microtubule lattice or microtubules ends that contact specific cortical sites to prevent stable and/or lateral attachments. Expand
Kinesin-13s in mitosis: Key players in the spatial and temporal organization of spindle microtubules.
TLDR
This review highlights recent findings that dissect the important actions of Kinesin-13 family members and summarizes important studies on the regulation of their activity by phosphorylation and by protein-protein interactions. Expand
Plus end-specific depolymerase activity of Kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle
TLDR
The role of Kip3p is explained in positioning the mitotic spindle in budding yeast and potentially other processes controlled by kinesin-8 family members, which are required for microtubule–cortical interactions, normal spindle assembly and kinetochore dynamics. Expand
The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends
TLDR
It is shown that MCAK moves along the microtubule lattice in a one-dimensional (1D) random walk, corresponding to a “reduction in dimensionality” that has been proposed as the search strategy by which DNA enzymes find specific binding sites. Expand
Kinesin-13 Regulates Flagellar, Interphase, and Mitotic Microtubule Dynamics in Giardia intestinalis
TLDR
It is shown that a single kinesin-13 homolog regulates flagellar length dynamics, as well as other interphase and mitotic dynamics in Giardia intestinalis, a widespread parasitic diplomonad protist, and suggested that axonemal microtubules are subject to the same regulation of microtubule dynamics as other dynamicmicrotubule arrays. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 48 REFERENCES
Yeast Bim1p Promotes the G1-specific Dynamics of Microtubules
TLDR
It is reported that a yeast protein of the highly conserved EB1 family, Bim1p, promotes cytoplasmic microtubule dynamics specifically during G1, where microtubules in cells lacking BIM1 showed reduced dynamicity due to a slower shrinking rate, fewer rescues and catastrophes, and more time spent in an attenuated/paused state. Expand
Cell cycle control of kinesin-mediated transport of Bik1 (CLIP-170) regulates microtubule stability and dynein activation.
TLDR
It is demonstrated that budding yeast Bik1, unlike its human ortholog CLIP-170, is targeted to the microtubule plus end by a kinesin-dependent transport mechanism, revealing a central role for Kip2- dependent transport in the cell cycle control of microtubules dynamics and dynein- dependent motility. Expand
An inner centromere protein that stimulates the microtubule depolymerizing activity of a KinI kinesin.
TLDR
By immunoelectron microscopy, it is found that ICIS is present on the surface of inner centromeres, placing it in an ideal location to depolymerize microtubules associated laterally with inner centromres and provide a mechanism that prevents kinetochore-microtubule attachment errors. Expand
Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle
TLDR
These results reveal crucial roles for EB1 in mitosis, which is postulate involves its ability to promote the growth and interactions of microtubules within the central spindle and at the cell cortex. Expand
Drosophila RhoGEF2 Associates with Microtubule Plus Ends in an EB1-Dependent Manner
TLDR
It is proposed that DRhoGEF2 uses microtubule dynamics to search for cortical subdomains of receptor-mediated Galpha activation, which in turn causes localized actomyosin contraction associated with morphogenetic movements during development. Expand
Targeted movement of cell end factors in fission yeast
TLDR
It is proposed that microtubule-based transport from the vicinity of the nucleus to cell ends can be precisely regulated, with Mal3 required for loading/processivity, Tea2 for movement and Tea1 for cell-end anchoring. Expand
Differentiation of cytoplasmic and meiotic spindle assembly MCAK functions by Aurora B-dependent phosphorylation.
TLDR
The results show that MCAK regulation of cytoplasmic and spindle-associated microtubules can be differentiated by Aurora B-dependent phosphorylation, and they further demonstrate that this regulation is required for bipolar meiotic spindle assembly. Expand
The microtubule-destabilizing kinesin XKCM1 regulates microtubule dynamic instability in cells.
TLDR
The results show that KCM1 plays a critical role in regulating both interphase and mitotic MT dynamics in mammalian cells and that perturbation of XKCM1 levels alters the catastrophe frequency and the rescue frequency of cellular MTs. Expand
The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly.
We have performed a mutational analysis together with RNA interference to determine the role of the kinesin-like protein KLP67A in Drosophila cell division. During both mitosis and male meiosis,Expand
Tea2p kinesin is involved in spatial microtubule organization by transporting tip1p on microtubules.
TLDR
This work shows that tea2p interacts with tip1p and that the two proteins move with high velocity along the microtubules toward their growing tips, suggesting a model in which kinesins regulate microtubule growth by transporting regulatory factors such as tip1P to the growing microtubULE tips. Expand
...
1
2
3
4
5
...