Functionalized two-photon absorbing diketopyrrolopyrrole-based fluorophores for living cells fluorescent microscopy.

@article{Ftouni2013FunctionalizedTA,
  title={Functionalized two-photon absorbing diketopyrrolopyrrole-based fluorophores for living cells fluorescent microscopy.},
  author={Hussein Ftouni and Fr{\'e}d{\'e}ric Bolze and Hugues de Rocquigny and J F Nicoud},
  journal={Bioconjugate chemistry},
  year={2013},
  volume={24 6},
  pages={942-50}
}
Two-photon excited microscopy has evolved as a routine technique for long-term cellular and in vivo imaging and is now available in most optical microscopy facilities. Classical dyes and fluorescent proteins, developed for epifluorescence or confocal microscopy, are used but unfortunately present a low efficiency upon two-photon excitation inducing the need of high excitation power (over 20 mW). To reduce this excitation power, new dyes need to be developed, allowing really low two-photon… CONTINUE READING