Functionalized membrane supports for covalent protein microsequence analysis.

@article{Coull1991FunctionalizedMS,
  title={Functionalized membrane supports for covalent protein microsequence analysis.},
  author={J. Coull and D. Pappin and J. Mark and R. Aebersold and H. K{\"o}ster},
  journal={Analytical biochemistry},
  year={1991},
  volume={194 1},
  pages={
          110-20
        }
}
Methods were developed for high yield covalent attachment of peptides and proteins to isothiocyanate and arylamine-derivatized poly(vinylidene difluoride) membranes for solid-phase sequence analysis. Solutions of protein or peptide were dried onto 8-mm membrane disks such that the functional groups on the surface and the polypeptide were brought into close proximity. In the case of the isothiocyanate membrane, reaction between polypeptide amino groups and the surface isothiocyanate moieties was… Expand
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