Functionalization of thioctic acid-capped gold nanoparticles for specific immobilization of histidine-tagged proteins.

@article{Abad2005FunctionalizationOT,
  title={Functionalization of thioctic acid-capped gold nanoparticles for specific immobilization of histidine-tagged proteins.},
  author={Jose Abad and Stijn F L Mertens and Marcos Pita and Victor Manuel Fernandez and David J. Schiffrin},
  journal={Journal of the American Chemical Society},
  year={2005},
  volume={127 15},
  pages={5689-94}
}
This paper presents an efficient strategy for the specific immobilization of fully functional proteins onto the surface of nanoparticles. Thioctic acid-derivatized gold clusters are used as a scaffold for further stepwise modification, leading to a cobalt(II)-terminated ligand shell. A histidine tag introduced by genetic engineering into a protein is coordinated to this transition metal ion. The specific immobilization has been demonstrated for the cases of a genetically engineered horseradish… CONTINUE READING