Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays.

  title={Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays.},
  author={F. Fixe and Martin Dufva and Pieter Telleman and Claus Bo V{\"o}ge Christensen},
  journal={Nucleic acids research},
  volume={32 1},
A chemical procedure was developed to functionalize poly(methyl methacrylate) (PMMA) substrates. PMMA is reacted with hexamethylene diamine to yield an aminated surface for immobilizing DNA in microarrays. The density of primary NH2 groups was 0.29 nmol/cm2. The availability of these primary amines was confirmed by the immobilization of DNA probes and hybridization with a complementary DNA strand. The hybridization signal and the hybridization efficiency of the chemically aminated PMMA slides… CONTINUE READING


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