Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment

@article{Ueki2008FunctionalTG,
  title={Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment},
  author={Shoko Ueki and Benoit V. Lacroix and Alexander Krichevsky and S. G. Lazarowitz and Vitaly Citovsky},
  journal={Nature Protocols},
  year={2008},
  volume={4},
  pages={71-77}
}
Transient gene expression is an indispensable tool for studying functions of gene products. In the case of plants, transient introduction of genes by Agrobacterium infiltration is a method of choice for many species. However, this technique does not work efficiently in Arabidopsis leaf tissue, the most widely used model system for basic plant biology research. Here we present an optimized protocol for biolistic delivery of plasmid DNA into the epidermis of Arabidopsis leaves, which can be… CONTINUE READING
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