Functional steps in transcription initiation and reinitiation from the major late promoter in a HeLa nuclear extract.

@article{Hawley1987FunctionalSI,
  title={Functional steps in transcription initiation and reinitiation from the major late promoter in a HeLa nuclear extract.},
  author={Diane K. Hawley and Robert G Roeder},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 8},
  pages={3452-61}
}
Transcription initiation by RNA polymerase II from the adenovirus major late promoter was studied in vitro in a nuclear extract derived from HeLa cells. We found that different concentrations of Sarkosyl blocked transcription initiation at several different functional steps. These corresponded to two steps defined previously in a partially purified transcription system: formation of the rapid start complex, which required incubation of template with the extract and was blocked by Sarkosyl… CONTINUE READING

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