• Corpus ID: 35888863

Functional states of RNA polymerase in the macronucleus of Tetrahymena pyriformis and their dependence on culture growth.

@article{Freiburg1981FunctionalSO,
  title={Functional states of RNA polymerase in the macronucleus of Tetrahymena pyriformis and their dependence on culture growth.},
  author={Manfred Freiburg},
  journal={Journal of cell science},
  year={1981},
  volume={47},
  pages={
          267-75
        }
}
  • M. Freiburg
  • Published 1 February 1981
  • Biology
  • Journal of cell science
RNA polymerase In in macronuclei of late log-phase cells of the ciliate Tetrahymena pyriformis is found to be present in 2 functional states, the one being actively engaged in transcription of ribosomal genes, the other one in a "free" state, i.e. free to transcribe added DNA or poly d(AT). Stimulation of RNA synthesis induced by dilution of stationary-phase cells into fresh medium is correlated with an increase in activity of template engaged and a decrease of free RNA polymerase I. In… 

Figures and Tables from this paper

Polysomal organization in growing and resting cultures and its relation to protein synthesis in Tetrahymena.
TLDR
The decrease in cellular RNA corresponded well with the reduction in amino acid incorporation in resting cells, suggesting that polypeptides in resting cultures are less stable.
Regulation of the maximal rate of RNA synthesis in the fission yeast Schizosaccharomyces pombe
  • S. Elliott
  • Biology
    Molecular and General Genetics MGG
  • 2004
TLDR
There is no correlation between transcription maximum and DNA synthesis or septation which suggests that these events do not exert a direct cell cycle control over RNA synthesis in exponentially growing cells.
Gene Action Changes during Nonvertebrate Differentiation
TLDR
A fuller picture of their nature and universality emerges from an examination of various life-history aspects of protistans, especially those of fungi and ciliates.
ISOLATION AND CHARACTERIZATION OF MACRONUCLEI OF PARAMECIUM CAUDATUM INFECTED WITH THE MACRONUCLEUS-SPECIFIC
TLDR
In all Holospora species two morphologically different forms can be distinguished, the long infectious form is taken up from the surrounding medium into a food vacuole and then transported into the nucleus that is specific to the species.
Toxicity and Mutagenicity of Patulin in Different Test Systems
TLDR
The mycotoxin reduces DNA synthesis by 50%, whereas RNA and protein synthesis are less inhibited than in the in vivo system, and an in vitro system could be developed consisting of permeabilized cells of Tetrahymena.
The Extent of Algal and Bacterial Endosymbioses in Protozoa1,2
TLDR
A survey is offered here of the whole range of such symbiotic associations (i.e. with species of protozoa serving as hosts) with the purposes of drawing attention to the exciting possibilities of such research and of reviewing significant findings made to date.
The heat shock response in Tetrahymena thermophila: control of protein synthesis
TLDR
No abnormalities were found in either heat shock or non-heat shock mRNA metabolism in starved cells unable to survive a sublethal heat shock when compared with the response of those cells which can survive such a treatment.

References

SHOWING 1-10 OF 26 REFERENCES
Changes in RNA Synthesis during the Culture Growth Cycle of Tetrahymena
TLDR
Evidence of a density-dependent inhibition of culture growth would indicate principles of growth control functioning in a similar way in unicellular organisms as observed in epithelial cells.
Regulation of RNA synthesis in Tetrahymena pyriformis: secretion of regulatory factors.
TLDR
Results show that the repression of RNA synthesis in densely populated cultures is caused by a dialysable factor, which is secreted by the cells, and if cells are inoculated on a medium which contains this factor the high initial rate ofRNA synthesis normally observed is prevented.
On the activity of RNA polymerase B in lysates from Ehrlich ascites cells.
TLDR
Transcription by endogenous RNA polymerase B in lysates of Ehrlich ascites cells was investigated and two functional states seem to be both elongating polymerase molecules.
NUCLEOLAR AND BIOCHEMICAL CHANGES DURING UNBALANCED GROWTH OF TETRAHYMENA PYRIFORMIS
TLDR
The involvement of unfused nucleoli in RNA and protein synthesis is suggested and the extreme plasticity of nucleoli with respect to changes in their environment is demonstrated.
Characterization of an Endogenous Transcription Inhibitor from Physarum polycephalum
TLDR
A substance purified from isolated nuclei of Physarum polycephalum by equilibrium and velocity gradient centrifugations, ion exchange chromatography and gel filtration which has a high molecular weight, is heat stable and resistant to amylases, proteases, nucleases and phosphodiesterase but is sensitive to phosphatases or hydrolysis.
Ribosome biosynthesis in Tetrahymena pyriformis. Regulation in response to nutritional changes
TLDR
It is estimated that the rate of ribosomal protein synthesis per cell increases at least 80-fold during the first 1-1.5 h after refeeding, reaching the level found in exponentially growing cells.
Modification of rat liver RNA polymerase I after in vivo stimulation by hydrocortisone or methylisobutylxanthine.
TLDR
The results indicate that in vivo stimulation by hydrocortisone or methylisobutylxanthine results in a conversion of the enzyme to a form that is catalytically more active but has an increased sensitivity to proteolysis.
...
1
2
3
...