Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis.

@article{He2010FunctionalSO,
  title={Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis.},
  author={Yan He and Bing Chen and Qiuying Pang and Johanna M Strul and Sixue Chen},
  journal={Plant & cell physiology},
  year={2010},
  volume={51 9},
  pages={1480-7}
}
The Arabidopsis genome encodes one potential isopropylmalate isomerase (IPMI) large subunit and three potential IPMI small subunits, which in bacteria and archaea form heterodimers to catalyze the isomerization of 2-isopropylmalate to 3-isopropylmalate in leucine biosynthesis. We demonstrate here that AtLeuC physically interacts with AtLeuD proteins to form functional IPMIs. The IPMIs are localized to chloroplast stroma. Tissue-specific expression analysis revealed that the patterns of AtLeuD1… CONTINUE READING