Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange.

@article{Maxwell2005FunctionalSB,
  title={Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange.},
  author={K. Maxwell and P. Reed and R. Zhang and S. Beasley and A. Walmsley and F. Curtis and A. Joachimiak and A. Edwards and G. Sharples},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 32},
  pages={
          11260-5
        }
}
  • K. Maxwell, P. Reed, +6 authors G. Sharples
  • Published 2005
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • Genetic recombination in bacteriophage lambda relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by beta protein. Phage lambda encodes an additional recombinase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein… CONTINUE READING
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