Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange.

@article{Maxwell2005FunctionalSB,
  title={Functional similarities between phage lambda Orf and Escherichia coli RecFOR in initiation of genetic exchange.},
  author={K. Maxwell and P. Reed and R. Zhang and S. Beasley and A. Walmsley and F. Curtis and A. Joachimiak and A. Edwards and G. Sharples},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 32},
  pages={
          11260-5
        }
}
  • K. Maxwell, P. Reed, +6 authors G. Sharples
  • Published 2005
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
    • Genetic recombination in bacteriophage lambda relies on DNA end processing by Exo to expose 3'-tailed strands for annealing and exchange by beta protein. Phage lambda encodes an additional recombinase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein… CONTINUE READING
    View on PubMed
    pnas.org
    23 Citations
    Highly Influencial Citations
    4
    Background Citations
    7

    Topics from this paper

    Explore Further: Topics Discussed in This Paper

    23 Citations
    Citation Type

    Citation Type

    The C‐terminus of the phage λ Orf recombinase is involved in DNA binding
    • 13
    Phage Orf Family Recombinases: Conservation of Activities and Involvement of the Central Channel in DNA Binding
    • 6
    • PDF
    Orf protein modulates phage and bacterial pathways of genetic recombination
    • 1
    DNA binding proteins of the phage λ ninR region
    • Highly Influenced
    Crystal structure of the Red beta C-terminal domain in complex with lambda Exonuclease reveals an unexpected homology with lambda Orf and an interaction with Escherichia coli single stranded DNA binding protein.
    • 6
    Physical Analyses of E. coli Heteroduplex Recombination Products In Vivo: On the Prevalence of 5′ and 3′ Patches
    • 4
    • PDF
    λ Recombination and Recombineering.
    • 22
    • PDF
    Structure and mechanism of the Red recombination system of bacteriophage λ.
    • 7
    The Protein Interaction Network of Bacteriophage Lambda with Its Host, Escherichia coli
    • 16
    • PDF
    Crystal structure of the Redβ C-terminal domain in complex with λ Exonuclease reveals an unexpected homology with λ Orf and an interaction with Escherichia coli single stranded DNA binding protein
    • 3
    • Highly Influenced
    • PDF

    References

    SHOWING 1-10 OF 44 REFERENCES
    The homologous recombination system of phage lambda. Pairing activities of beta protein.
    • 128
    What makes the bacteriophage λ Red system useful for genetic engineering: molecular mechanism and biological function
    • 114
    • PDF
    Purification and preliminary characterization of the Escherichia coli K-12 recF protein.
    • 59
    • PDF
    Protein interactions in genetic recombination in Escherichia coli. Interactions involving RecO and RecR overcome the inhibition of RecA by single-stranded DNA-binding protein.
    • 243
    Purification and characterization of the Escherichia coli RecO protein. Renaturation of complementary single-stranded DNA molecules catalyzed by the RecO protein.
    • 77
    Modulation of DNA Repair and Recombination by the Bacteriophage λ Orf Function in Escherichia coli K-12
    • 19
    • PDF
    Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination
    • 132
    • PDF
    The phage lambda orf gene encodes a trans-acting factor that suppresses Escherichia coli recO, recR, and recF mutations for recombination of lambda but not of E. coli.
    • 29
    • PDF
    Homologous pairing of single-stranded DNA and superhelical double-stranded DNA catalyzed by RecO protein from Escherichia coli.
    • 42
    • PDF
    Strand invasion promoted by recombination protein β of coliphage λ
    • 42
    • PDF