Functional screening yields a new beta-1,4-endoglucanase gene from Heterodera glycines that may be the product of recent gene duplication.

@article{Yan2001FunctionalSY,
  title={Functional screening yields a new beta-1,4-endoglucanase gene from Heterodera glycines that may be the product of recent gene duplication.},
  author={Yitang Yan and Geert Smant and Eric Davis},
  journal={Molecular plant-microbe interactions : MPMI},
  year={2001},
  volume={14 1},
  pages={63-71}
}
Clones with secreted cellulolytic activity were identified when a cDNA library constructed from poly A(+) RNA of preparasitic second-stage juveniles of Heterodera glycines, the soybean cyst nematode, was expressed in the Escherichia coli SOLR strain and overlaid with a carboxymethylcellulose (CMC) substrate. Twenty CMC-degrading clones were analyzed, and all were either identical or strongly similar to a beta-1,4-endoglucanase gene (HG-eng-2), previously isolated from H. glycines. A subgroup of… CONTINUE READING

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The highly conserved nucleotide sequence in the introns and exons and their close proximity within the genome suggest that HG - eng-2 and HG - eng-3 are the products of recent gene duplication and inversion .
IntronsNo subtypeExons
The highly conserved nucleotide sequence in the introns and exons and their close proximity within the genome suggest that HG - eng-2 and HG - eng-3 are the products of recent gene duplication and inversion .
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