Functional roles of the 6-S-cysteinyl, 8alpha-N1-histidyl FAD in glucooligosaccharide oxidase from Acremonium strictum.

@article{Huang2008FunctionalRO,
  title={Functional roles of the 6-S-cysteinyl, 8alpha-N1-histidyl FAD in glucooligosaccharide oxidase from Acremonium strictum.},
  author={Chun-hsiang Huang and Andreas Winkler and Chia-Lin Chen and Wen-Lin Lai and Yu-C. Tsai and Peter Macheroux and Shu Hwang Liaw},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 45},
  pages={
          30990-6
        }
}
The crystal structure of glucooligosaccharide oxidase from Acremonium strictum was demonstrated to contain a bicovalent flavinylation, with the 6- and 8alpha-positions of the flavin isoalloxazine ring cross-linked to Cys(130) and His(70), respectively. The H70A and C130A single mutants still retain the covalent FAD, indicating that flavinylation at these two residues is independent. Both mutants exhibit a decreased midpoint potential of approximately +69 and +61 mV, respectively, compared with… CONTINUE READING

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IT Y on M arch 7, 2015 hp://w w w .jb.org/ D

  • R. H. van den Heuvel, M. W. Fraaije, A. Mattevi, W. J. van Berkel
  • J. Biol
  • 2000
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