Functional roles of TRPV1 and TRPV4 in control of lower urinary tract activity: dual analysis of behavior and reflex during the micturition cycle.

  title={Functional roles of TRPV1 and TRPV4 in control of lower urinary tract activity: dual analysis of behavior and reflex during the micturition cycle.},
  author={Mitsuharu Yoshiyama and Tsutomu Mochizuki and Hiroshi Nakagomi and Tatsuya Miyamoto and Satoru Kira and Ryoji Mizumachi and Takaaki Sokabe and Yasunori Takayama and Makoto Tominaga and Masayuki Takeda},
  journal={American journal of physiology. Renal physiology},
  volume={308 10},
The present study used a dual analysis of voiding behavior and reflex micturition to examine lower urinary tract function in transient receptor potential vanilloid (TRPV)1 knockout (KO) mice and TRPV4 KO mice. In metabolic cage experiments conducted under conscious conditions (i.e., voluntary voiding behavior), TRPV4 KO mice showed a markedly higher voiding frequency (VF; 19.3 ± 1.2 times/day) and a smaller urine volume/voiding (UVV; 114 ± 9 μl) compared with wild-type (WT) littermates (VF: 5.2… 

Figures and Tables from this paper

TRPV4 blockade reduces voiding frequency, ATP release and pelvic sensitivity in mice with chronic urothelial overexpression of NGF (NGF-OE).

It is suggested that TRPV4 contributes to luminal ATP release from the urinary bladder, increased voiding frequency and pelvic sensitivity in NGF-OE mice.

Reduced bladder responses to capsaicin and GSK-1016790A in retired-breeder female rats with diminished volume sensitivity.

The results suggest that RB rats have a decreased bladder sensory function commonly observed in elderly women, and could be used as an animal model to study the underling mechanisms.

TRP Channels as Lower Urinary Tract Sensory Targets

The development of selective TRP channel agonists and antagonists is ongoing and the use of such agents can be expected to offer new and important information concerning both normal physiological functions and possible therapeutic applications.

Blockade of Acid-Sensing Ion Channels Increases Urinary Bladder Capacity With or Without Intravesical Irritation in Mice

It is suggested that blockade of ASIC signal transduction increases bladder capacity under normal intravesical pH conditions and alleviates bladder hyperreflexia induced by intravesicals acidification and that the site responsible for this action is likely to be the dorsal root ganglia.

Emerging roles of the TRPV4 channel in bladder physiology and dysfunction

Emerging evidence indicates that the TRPV4 channel plays a sensory role in the uroepithelium, where it may regulate the release of sensory mediators such as ATP, which in turn modulates afferent nerve activity in response to bladder filling during the urination cycle.

Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior

Findings indicate that urothelial Cx43 mediated ATP signaling and coordination of u rothelial activity are essential for proper perception and regulation of responses to bladder distension in the animals’ awake, active phase.

Transient receptor potential channels in sensory mechanisms of the lower urinary tract

The involvement of TRP channels in normal and abnormal bladder sensations and their potential as therapeutic targets are discussed and translation to clinical applications remains limited.

Structure, function, and mechanism of action of the vanilloid TRPV1 receptor

The TRPV1 receptor (transient receptor potential cation channel subfamily V member 1) is a non-selective cationic channel activated by vanilloids like capsaicin, which is a spicy substance found in chili peppers.

Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia.

TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying and is mapped to Ca(2+)-imaging and electrophysiology.




Detailed evaluations using in-vivo cystometrograms in decerebrate unanaesthetized mice facilitated determining the possible involvement of TRPV4 in the underlying mechanism.

Deletion of the transient receptor potential cation channel TRPV4 impairs murine bladder voiding.

Evidence for a critical role of the transient receptor potential cation channel, subfamily V, member 4 (TRPV4) in normal bladder function is provided and a previously unrecognized role for TRpV4 in voiding behavior is demonstrated, raising the possibility that TRPV 4 plays acritical role in urothelium-mediated transduction of intravesical mechanical pressure.

Inhibition of the cation channel TRPV4 improves bladder function in mice and rats with cyclophosphamide-induced cystitis

It is shown that the development of cystitis-induced bladder dysfunction is strongly impaired in Trpv4−/− mice, and HC-067047 is described, a previously uncharacterized, potent, and selective TRPV4 antagonist that increases functional bladder capacity and reduces micturition frequency in WT mice and rats with Cystitis.

Where is TRPV1 expressed in the bladder, do we see the real channel?

These data show that TRPV1 antibodies can cause an aspecific immunostaining in bladder tissue, and think that the use of negative controls on knockout mice, whenever available, is mandatory when conducting immunohistochemical localization studies.

TRPV1 (vanilloid receptor) in the urinary tract: expression, function and clinical applications

It is now increasingly clear that TRPV1 also regulates the frequency of bladder reflex contractions, either through direct excitation of sensory fibers or through urothelial-sensory fiber cross talk involving the release of neuromediators from the epithelial cells.

Effects of urethane on reflex activity of lower urinary tract in decerebrate unanesthetized rats.

The results suggest that urethral activity, which is essential for efficient voiding, is more sensitive to the suppressive effect of urethane than afferent or efferent mechanisms controlling the bladder.