Functional reconstitution of the human chemokine receptor CXCR4 with Gi/Go-proteins in Sf9 insect cells

@article{Kleemann2008FunctionalRO,
  title={Functional reconstitution of the human chemokine receptor CXCR4 with Gi/Go-proteins in Sf9 insect cells},
  author={Patrick Kleemann and Dan Papa and Sandy Vigil-Cruz and Roland Seifert},
  journal={Naunyn-Schmiedeberg's Archives of Pharmacology},
  year={2008},
  volume={378},
  pages={261-274}
}
The chemokine stromal cell-derived factor-1α (SDF-1α) binds to the chemokine receptor CXCR4 that couples to pertussis toxin-sensitive G-proteins of the Gi/Go-family. CXCR4 plays a role in the pathogenesis of autoimmune diseases, human immunodeficiency virus infection and various tumors, fetal development as well as endothelial progenitor and T-cell recruitment. To this end, most CXCR4 studies have focused on the cellular level. The aim of this study was to establish a reconstitution system for… CONTINUE READING
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