Functional reconstitution of influenza virus envelopes.

@article{Stegmann1987FunctionalRO,
  title={Functional reconstitution of influenza virus envelopes.},
  author={Toon Stegmann and Henri{\"e}tte W. M. Morselt and Frank P. Booy and J F van Breemen and Gl Scherphof and Jan Wilschut},
  journal={The EMBO journal},
  year={1987},
  volume={6 9},
  pages={
          2651-9
        }
}
We have examined several procedures for the reconstitution of influenza virus envelopes, based on detergent removal from solubilized viral membranes. With octylglucoside, no functionally active virosomes are formed, irrespective of the rate of detergent removal: in the final preparation the viral spike proteins appear predominantly as rosettes. Protein incorporation in reconstituted vesicles is improved when a method based on reverse-phase evaporation of octylglucoside-solubilized viral… CONTINUE READING
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