Functional reconstitution of cell-free synthesized purified Kv channels.

@article{Renauld2017FunctionalRO,
  title={Functional reconstitution of cell-free synthesized purified Kv channels.},
  author={St{\'e}phane Renauld and Sandra Cort{\'e}s and Beate Bersch and Xavier Henry and Michel De Waard and B{\'e}atrice Schaack},
  journal={Biochimica et biophysica acta},
  year={2017},
  volume={1859 12},
  pages={2373-2380}
}
The study of ion channel activity and the screening of possible inhibitor molecules require reliable methods for production of active channel proteins, their insertion into artificial membranes and for the measurement of their activity. Here we report on cell-free expression of soluble and active Kv1.1 and Kv1.3 channels and their efficient insertion into… CONTINUE READING