Functional receptors for epidermal growth factor in an epithelial-cell line derived from the rat small intestine.

@article{Blay1985FunctionalRF,
  title={Functional receptors for epidermal growth factor in an epithelial-cell line derived from the rat small intestine.},
  author={Jonathan Blay and Kevin Brown},
  journal={The Biochemical journal},
  year={1985},
  volume={225 1},
  pages={85-94}
}
Epidermal growth factor (EGF) regulates the proliferation of cells of a rat intestinal epithelial-cell line (RIE-1) in culture. Confluent RIE-1 cells were stimulated to proliferate by EGF with a half-maximal effect at 1-2 ng/ml. In contrast, the growth of sparse RIE-1 cells was inhibited by the growth factor. Binding studies at 4 degrees C with 125I-EGF identified two classes of binding sites for EGF on RIE-1 cells, one of high affinity (KD = 1.8 X 10(-10)M; 1.8 X 10(4) receptors/cell) and one… CONTINUE READING
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