Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth.

@article{Zhang2015FunctionalPF,
  title={Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth.},
  author={Yiming Zhang and Zongjie Dai and Anastasia Krivoruchko and Yun Chen and Verena Siewers and Jens B Nielsen},
  journal={FEMS yeast research},
  year={2015},
  volume={15 4},
  pages={fov024}
}
Pyruvate formate lyase (PFL) is characterized as an enzyme functional at anaerobic conditions, since the radical in the enzyme's active form is sensitive to oxygen. In this study, PFL and its activating enzyme from Escherichia coli were expressed in a Saccharomyces cerevisiae strain lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1, IMI076 (Pdc(-) MTH1-ΔT ura3-52). PFL was expressed with two different electron donors… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 34 references

Engineering a synthetic dual-organism system for hydrogen production.

Applied and environmental microbiology • 2009
View 6 Excerpts
Highly Influenced

Replacement of the Saccharomyces cerevisiae acetyl-CoA synthetases by alternative pathways for cytosolic acetyl-CoA synthesis.Metab Eng 2014a;21:46–59

BU Kozak, HM van Rossum, KR Benjamin
2014
View 6 Excerpts
Highly Influenced

Enhancing the copy number

S Partow, G Scalcinati
Curr Opin Biotechnol • 2013

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