Functional proteomic screens reveal an essential extracellular role for hsp90α in cancer cell invasiveness

@article{Eustace2004FunctionalPS,
  title={Functional proteomic screens reveal an essential extracellular role for hsp90$\alpha$ in cancer cell invasiveness},
  author={Brenda K. Eustace and Takashi Sakurai and Jean Ko Stewart and Dean Yimlamai and Christine Unger and Carol Zehetmeier and B Santiago La{\'i}n and Claudia Torella and Stefan W. Henning and Gerald Beste and Bradley T. Scroggins and Len Neckers and Leodevico L Ilag and Daniel G. Jay},
  journal={Nature Cell Biology},
  year={2004},
  volume={6},
  pages={507-514}
}
Tumour cell invasiveness is crucial for cancer metastasis and is not yet understood. Here we describe two functional screens for proteins required for the invasion of fibrosarcoma cells that identified the molecular chaperone heat shock protein 90 (hsp90). The hsp90α isoform, but not hsp90β, is expressed extracellularly where it interacts with the matrix metalloproteinase 2 (MMP2). Inhibition of extracellular hsp90α decreases both MMP2 activity and invasiveness. This role for extracellular… 
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