Functional properties of the apical Na+-K+-2Cl- cotransporter isoforms.

  title={Functional properties of the apical Na+-K+-2Cl- cotransporter isoforms.},
  author={Consuelo Plata and Patricia Meade and Norma V{\'a}zquez and Steven C. Hebert and Gerardo Gamba},
  journal={The Journal of biological chemistry},
  volume={277 13},
The bumetanide-sensitive Na(+):K(+):2Cl(-) cotransporter (BSC1) is the major pathway for salt reabsorption in the apical membrane of the mammalian thick ascending limb of Henle. Three isoforms of the cotransporter, known as A, B, and F, exhibit axial expression along the thick ascending limb. We report here a functional comparison of the three isoforms from mouse kidney. When expressed in Xenopus oocytes the mBSC1-A isoform showed higher capacity of transport, with no difference in the amount… CONTINUE READING