Functional organization of the yeast proteome by systematic analysis of protein complexes

@article{Gavin2002FunctionalOO,
  title={Functional organization of the yeast proteome by systematic analysis of protein complexes},
  author={Anne-Claude Gavin and Markus B{\"o}sche and Roland Krause and Paola Grandi and Martina Marzioch and Andreas Bauer and J{\"o}rg Schultz and Jens M. Rick and Anne Marie Michon and C M Cruciat and Marita Remor and Christian H{\"o}fert and Malgorzata Schelder and Miro Brajenovic and Heinz Ruffner and Alejandro Merino and Karin Klein and Manuela Hudak and David Dickson and Tatjana Rudi and Volker Gnau and Angela Bauch and Sonja Bastuck and Bettina Huhse and Christina Leutwein and Marie-Anne Heurtier and Richard R. Copley and Angela Edelmann and Erich Querfurth and Vladimir Rybin and Gerard Drewes and Manfred Raida and Tewis Bouwmeester and Peer Bork and Bertrand S{\'e}raphin and Bernhard Kuster and Gitte Neubauer and Giulio Superti-Furga},
  journal={Nature},
  year={2002},
  volume={415},
  pages={141-147}
}
Most cellular processes are carried out by multiprotein complexes. The identification and analysis of their components provides insight into how the ensemble of expressed proteins (proteome) is organized into functional units. We used tandem-affinity purification (TAP) and mass spectrometry in a large-scale approach to characterize multiprotein complexes in Saccharomyces cerevisiae. We processed 1,739 genes, including 1,143 human orthologues of relevance to human biology, and purified 589… Expand

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