Functional neutrophils from long-term murine bone marrow cell cultures.

@article{Hart1987FunctionalNF,
  title={Functional neutrophils from long-term murine bone marrow cell cultures.},
  author={Prue H Hart and Leslie Spencer and Paraic A. Kenny and Angel F Lopez and Peter J. McDonald and John J. Finlay-Jones},
  journal={Journal of immunological methods},
  year={1987},
  volume={100 1-2},
  pages={223-33}
}
Murine bone marrow cell cultures that had been established for up to 26 weeks were harvested each week and found to provide functional neutrophils. Leukocytes harvested from the cultures were enriched for neutrophils using discontinuous Percoll density gradients. These cells mounted a chemiluminescence response to Proteus mirabilis in the presence of normal… CONTINUE READING