Functional muscarinic M2 and M3 receptors and beta-adrenoceptor in cultured rat bladder smooth muscle.

@article{Ma2002FunctionalMM,
  title={Functional muscarinic M2 and M3 receptors and beta-adrenoceptor in cultured rat bladder smooth muscle.},
  author={Fu Hai Ma and Hanae Higashira-Hoshi and Yoshinori Itoh},
  journal={Life sciences},
  year={2002},
  volume={70 10},
  pages={
          1159-72
        }
}
A highly purified rat urinary bladder smooth muscle cell culture was obtained by a modified enzymic isolation method, and the presence of functional muscarinic as well as beta-adrenergic receptors were subsequently determined. At 7-10 days of culture, cells became elongated and spindle-shaped showing a typical "hills and valleys" form. They were stained with anti-alpha-actin and anti-myosin antibodies. Radiolabeled ligand binding using [3H]N-methylscopolamine and [3H]CGP12177 showed that these… CONTINUE READING

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