Functional mapping of an entomocidal delta-endotoxin. Single amino acid changes produced by site-directed mutagenesis influence toxicity and specificity of the protein.

Abstract

Mutagenesis has been used to investigate the toxicity and specificity of a larvicidal protein from Bacillus thuringiensis aizawai IC1 that is toxic to both lepidoptera and diptera and differs by only three residues from a monospecific lepidopteran toxin from B. thuringiensis berliner. Site-directed mutagenesis was used to investigate the contribution of… (More)

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