Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center.

@article{Siatecka1999FunctionalIO,
  title={Functional interactions of Prp8 with both splice sites at the spliceosomal catalytic center.},
  author={Miroslawa Siatecka and J. B. de los Reyes and Maria M. Konarska},
  journal={Genes & development},
  year={1999},
  volume={13 15},
  pages={1983-93}
}
A U5 snRNP protein, hPrp8, interacts closely with the GU dinucleotide at the 5' splice site (5'SS), forming a specific UV-inducible cross-link. To test if this physical contact between the 5'SS and the carboxy-terminal region of Prp8 reflects a functional recognition of the 5'SS during spliceosome assembly, we mutagenized the corresponding region of yeast Prp8 and screened the resulting mutants for suppression of 5'SS mutations in vivo. All of the isolated prp8 alleles not only suppress 5'SS… CONTINUE READING