Functional interactions between the G' subdomain of bacterial translation factor EF-G and ribosomal protein L7/L12.

@article{Nechifor2007FunctionalIB,
  title={Functional interactions between the G' subdomain of bacterial translation factor EF-G and ribosomal protein L7/L12.},
  author={Roxana Nechifor and Marat B. Murataliev and Kevin Scott Wilson},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 51},
  pages={36998-7005}
}
Protein L7/L12 of the bacterial ribosome plays an important role in activating the GTP hydrolytic activity of elongation factor G (EF-G), which promotes ribosomal translocation during protein synthesis. Previously, we cross-linked L7/L12 from two residues (209 and 231) flanking alpha-helix AG' in the G' subdomain of Escherichia coli EF-G. Here we report kinetic studies on the functional effects of mutating three neighboring glutamic acid residues (224, 228, and 231) to lysine, either singly or… CONTINUE READING

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