Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics

@article{Carter2000FunctionalIF,
  title={Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics},
  author={Andrew Pearce Carter and William M. Clemons and Ditlev Egeskov Brodersen and Robert J. Morgan-Warren and Brian T. Wimberly and Venki Ramakrishnan},
  journal={Nature},
  year={2000},
  volume={407},
  pages={340-348}
}
The 30S ribosomal subunit has two primary functions in protein synthesis. It discriminates against aminoacyl transfer RNAs that do not match the codon of messenger RNA, thereby ensuring accuracy in translation of the genetic message in a process called decoding. Also, it works with the 50S subunit to move the tRNAs and associated mRNA by precisely one codon, in a process called translocation. Here we describe the functional implications of the high-resolution 30S crystal structure presented in… 

Atomic structures of the 30S subunit and its complexes with ligands and antibiotics.

The two subunits that make up the ribosome have both distinct and cooperative functions. The 30S ribosomal subunit binds messenger RNA (mRNA) and is involved in the selection of cognate transfer RNA

Structure of the 30S ribosomal subunit

The crystal structure of the 30S subunit from Thermus thermophilus, refined to 3 Å resolution, is reported, which will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography.

Structural Studies of the Functional Complexes of the 50S and 70S Ribosome, a Major Antibiotic Target

Our crystal structure of the Haloarcula marismortui (H.ma.) 50S ribosomal subunit and its complexes with substrates and antibiotics have illuminated the mechanism of peptide bond formation and its

From the structure and function of the ribosome to new antibiotics

The crystal structures of the 70S ribosome bound to two compounds that are effective against tuberculosis, capreomycin and viomycin are determined and the design of new anti-TB antibiotics by chemically combining components of the neighboring compounds should be possible.

Structural studies on decoding, termination and translocation in the bacterial ribosome

This chapter reviews studies on decoding using the 30S subunit, which could use crystals of the subunit that diffracted to high resolution, but other functional studies such as those on elongation and termination required new crystal forms of the 70S ribosome that also diffraction to high-resolution.

Codon-Anticodon Interaction at the P Site Is a Prerequisite for tRNA Interaction with the Small Ribosomal Subunit*

It is demonstrated that a tRNA bound to the P site of non-programmed 70 S ribosomes contacts predominantly the 50 S, as opposed to the 30 S subunit, indicating that codon-anticodon interaction at the P sites is a prerequisite for 30 S binding.

Ribosomal crystallography: peptide bond formation and its inhibition.

The rotatory motion is the major component of unified machinery for peptide-bond formation, translocation, and nascent protein progression, since its spiral nature ensures the entrance of the nascent peptide into the ribosomal exit tunnel.
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The crystal structure of the 30S subunit from Thermus thermophilus, refined to 3 Å resolution, is reported, which will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography.

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