Functional implication with the metal-binding properties of KChIP1.

  title={Functional implication with the metal-binding properties of KChIP1.},
  author={Long-sen Chang and Chia-yi Chen and Tony Tong-lin Wu},
  journal={Biochemical and biophysical research communications},
  volume={311 2},
To investigate the metal-binding properties of KChIP1, the interaction of KChIP1 and mutated KChIP1 with divalent cations (Mg(2+), Ca(2+), Sr(2+), and Ba(2+)) was explored by 8-anilinonaphthalene-1-sulfonate (ANS) fluorescence. It showed that KChIP1 possessed two types of Ca(2+)-binding sites, high-affinity and low-affinity Ca(2+)-binding sites. However, only low-affinity-binding site for Mg(2+), Sr(2+), and Ba(2+) was observed. The metal-binding properties of KChIP1 are not appreciably… CONTINUE READING