Functional immobilization of signaling proteins enables control of stem cell fate

  title={Functional immobilization of signaling proteins enables control of stem cell fate},
  author={Kristin Alberti and Ryan E. Davey and Kento Onishi and Sophia H. L. George and Katrin Salchert and F Philipp Seib and Martin Bornhaeuser and Tilo Pompe and Andras D Nagy and Carsten Werner and Peter W. Zandstra},
  journal={Nature Methods},
The mode of ligand presentation has a fundamental role in organizing cell fate throughout development. We report a rapid and simple approach for immobilizing signaling ligands to maleic anhydride copolymer thin-film coatings, enabling stable signaling ligand presentation at interfaces at defined concentrations. We demonstrate the utility of this platform technology using leukemia inhibitory factor (LIF) and stem cell factor (SCF). Immobilized LIF supported mouse embryonic stem cell (mESC… CONTINUE READING


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the OP9–DL1 approach

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