Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase.

@article{Sterling2006FunctionalIO,
  title={Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase.},
  author={Jason Dwight Sterling and Melani A Atmodjo and Sarah E. Eichler Inwood and V S Kumar Kolli and Heather F Quigley and Michael G Hahn and Debra Mohnen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2006},
  volume={103 13},
  pages={
          5236-41
        }
}
Galacturonosyltransferases (GalATs) are required for the synthesis of pectin, a family of complex polysaccharides present in the cell walls of all land plants. We report the identification of a pectin GalAT (GAUT1) using peptide sequences obtained from Arabidopsis thaliana proteins partially purified for homogalacturonan (HG) alpha-1,4-GalAT activity. Transient expression of GAUT1 cDNA in the human embryonic kidney cell line HEK293 yielded uridine diphosphogalacturonic acid:GalAT activity… CONTINUE READING

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