Functional identification of an 8-oxoguanine specific endonuclease from Thermotoga maritima.

@article{Im2005FunctionalIO,
  title={Functional identification of an 8-oxoguanine specific endonuclease from Thermotoga maritima.},
  author={Eun Kyoung Im and Chang Hyung Hong and Jung Ho Back and Ye Sun Han and Ji Hyung Chung},
  journal={Journal of biochemistry and molecular biology},
  year={2005},
  volume={38 6},
  pages={676-82}
}
To date, no 8-oxoguanine-specific endonuclease-coding gene has been identified in Thermotoga maritima of the order Thermotogales, although its entire genome has been deciphered. However, the hypothetical protein Tm1821 from T. maritima, has a helix-hairpin-helix motif that is considered to be important for DNA binding and catalytic activity. Here, Tm1821 was overexpressed in Escherichia coli and purified using Ni-NTA affinity chromatography, protease digestion, and gel filtration. Tm1821… CONTINUE READING