Functional homologs of cyanovirin-N amenable to mass production in prokaryotic and eukaryotic hosts.

@article{Mori2002FunctionalHO,
  title={Functional homologs of cyanovirin-N amenable to mass production in prokaryotic and eukaryotic hosts.},
  author={Toshiyuki Mori and Laura G. Barrientos and Zhaozhong Han and Angela M. Gronenborn and Jim A. Turpin and Michael R. Boyd},
  journal={Protein expression and purification},
  year={2002},
  volume={26 1},
  pages={
          42-9
        }
}
Cyanovirin-N (CV-N) is under development as a topical (vaginal or rectal) microbicide to prevent sexual transmission of human immunodeficiency virus (HIV), and an economically feasible means for very large-scale production of the protein is an urgent priority. We observed that N-glycosylation of CV-N in yeast eliminated the anti-HIV activity, and that dimeric forms and aggregates of CV-N occurred under certain conditions, potentially complicating the efficient, large-scale manufacture of pure… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 23 CITATIONS

Soluble cytoplasmic expression, rapid purification, and characterization of cyanovirin-N as a His-SUMO fusion

  • Applied Microbiology and Biotechnology
  • 2009
VIEW 5 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Unexpected synergistic HIV neutralization by a triple microbicide produced in rice endosperm

  • Proceedings of the National Academy of Sciences of the United States of America
  • 2018

Cyanobacterial lectins characteristics and their role as antiviral agents.

  • International journal of biological macromolecules
  • 2017
VIEW 2 EXCERPTS
CITES METHODS