Functional gene discovery using hybrid ribozyme libraries.

  title={Functional gene discovery using hybrid ribozyme libraries.},
  author={Yoshio Kato and Masaru Tsunemi and Makoto Miyagishi and Hiroaki Kawasaki and K. Taira},
  journal={Methods in molecular biology},
Hybrid ribozymes that couple the cleavage activity of hammerhead ribozymes with the unwinding activity of RNA helicases are powerful tools in the study of cell genetics and pharmaceutical drug development. They are useful for targeting a specific gene as well as screening functional genes to show phenotypic alterations. By randomizing the binding arms within the ribozymes, we can create a library of ribozymes that are capable of cleaving any mRNA. After introducing the library into cells and… CONTINUE READING


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