Functional expression of the mutants of the chloroplast tRNA(Lys) gene from the liverwort, Marchantia polymorpha, in Escherichia coli.

@article{Nakahigashi1990FunctionalEO,
  title={Functional expression of the mutants of the chloroplast tRNA(Lys) gene from the liverwort, Marchantia polymorpha, in Escherichia coli.},
  author={Kenji Nakahigashi and Hachiro Inokuchi and Haruo Ozeki},
  journal={FEBS letters},
  year={1990},
  volume={265 1-2},
  pages={59-62}
}
The anticodon of the tRNA(Lys) gene (trnK) in the liverwort, Marchantia polymorpha, was artificially converted to an amber anticodon. This mutant tRNA(Lys) (CTA) gene carrying either the intron of the C27-C43 mismatch at the anticodon-stem is not functional in Escherichia coli, but without both of them, it does work as a tRNA(Lys) amber suppressor.