Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris.

@article{SoaresSilva2003FunctionalEO,
  title={Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris.},
  author={Isabel Soares-Silva and Dorit Schuller and Raquel P. Andrade and F{\'a}tima Baltazar and Fernanda C{\'a}ssio and Margarida Casal},
  journal={The Biochemical journal},
  year={2003},
  volume={376 Pt 3},
  pages={781-7}
}
In Saccharomyces cerevisiae the activity for the lactate-proton symporter is dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western blotting analyses indicated the expression of JEN1 in the transformants. Lactate permease activity was obtained in P. pastoris cells with a V (max) of 2.1 nmol x s(-1) x mg of dry weight(-1). Reconstitution of the lactate permease activity was… CONTINUE READING

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