Functional expression of the genes of Escherichia coli in gram-positive Corynebacterium glutamicum

@article{Ozaki1984FunctionalEO,
  title={Functional expression of the genes of Escherichia coli in gram-positive Corynebacterium glutamicum},
  author={Akio Ozaki and Ryoichi Katsumata and Tetsuo Oka and Akira Furuya},
  journal={Molecular and General Genetics MGG},
  year={1984},
  volume={196},
  pages={175-178}
}
Hybrid plasmids were constructed by combining in vitro the Escherichia coli plasmid pGA22, which carries the genes determining resistance to kanamycin, tetracycline, chloramphenicol and ampicillin, with the cryptic plasmids, pCG1 and pCG2, of Corynebacterium glutamicum. The hybrid plasmids were introduced into C. glutamicum and E. coli and replicated in both hosts. They expressed all the E. coli resistance phenotypes except ampicillin resistance in C. glutamicum. The levels of antibiotic… CONTINUE READING