Functional expression of recombinant N-methyl-D-aspartate receptors in the yeast Saccharomyces cerevisiae--localization and pharmacological characterization.

@article{Li1998FunctionalEO,
  title={Functional expression of recombinant N-methyl-D-aspartate receptors in the yeast Saccharomyces cerevisiae--localization and pharmacological characterization.},
  author={Z. Li and J. Becker and C. Noe},
  journal={European journal of biochemistry},
  year={1998},
  volume={252 3},
  pages={
          391-9
        }
}
  • Z. Li, J. Becker, C. Noe
  • Published 1998
  • Biology, Medicine
  • European journal of biochemistry
  • The yeast Saccharomyces cerevisiae was used for expressing the genes encoding the ionotropic N-methyl-D-aspartate (NMDA) receptor subunits from rats (NR1a, NR2A, NR2C) and mice (NR2B). Four plasmids were constructed by cloning the different NMDA receptor genes in the two multi-copy yeast-Escherichia coli shuttle vectors pMB01 (--> NR1a gene) and pMB02 (--> NR2A-2C genes). The protease-deficient S. cerevisiae strain cI3-ABYS-86 (leu-, ura-) was transformed or co-transformed with the resulting… CONTINUE READING
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