Functional expression of hepassocin in Escherichia coli using SUMO fusion partner and molecular chaperones.

@article{Wang2013FunctionalEO,
  title={Functional expression of hepassocin in Escherichia coli using SUMO fusion partner and molecular chaperones.},
  author={Qi Wang and Jiaojiao Zhao and Yan Wang and Honglou Sun and Yi Jiang and Lan Luo and Zhimin Yin},
  journal={Protein expression and purification},
  year={2013},
  volume={92 2},
  pages={135-40}
}
Human hepassocin (HPS) is a hepatic growth factor which can accelerate hepatocyte proliferation in vivo and protect against liver injury. Previous reports have shown that HPS expressed in Escherichia coli resulted in inclusion bodies or low yield. In this study, the application of small ubiquitin-related modifier (SUMO) fusion technology in combined with four different chaperone teams on the soluble expression of recombinant HPS protein were explored and analyzed. The soluble expression of HPS… CONTINUE READING

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