Functional expression of a thermophilic glucuronoyl esterase from Sporotrichum thermophile: identification of the nucleophilic serine

@article{Topakas2010FunctionalEO,
  title={Functional expression of a thermophilic glucuronoyl esterase from Sporotrichum thermophile: identification of the nucleophilic serine},
  author={Evangelos Topakas and Maria Moukouli and Maria Dimarogona and Christina Vafiadi and Paul Christakopoulos},
  journal={Applied Microbiology and Biotechnology},
  year={2010},
  volume={87},
  pages={1765-1772}
}
A glucuronoyl esterase (GE) from the thermophilic fungus Sporotrichum thermophile, belonging to the carbohydrate esterase family 15 (CE-15), was functionally expressed in the methylotrophic yeast Pichia pastoris. The putative GE gene ge2 from the genomic DNA was successfully cloned in frame with the sequence for the Saccharomyces cerevisiae α-factor secretion signal under the transcriptional control of the alcohol oxidase (AOX1) promoter and integrated in P. pastoris X-33 to confirm that the… CONTINUE READING

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