Functional expression of a sequence-specific endonuclease encoded by the retrotransposon R2Bm.

@article{Xiong1988FunctionalEO,
  title={Functional expression of a sequence-specific endonuclease encoded by the retrotransposon R2Bm.},
  author={Yuanyan Xiong and Thomas H. Eickbush},
  journal={Cell},
  year={1988},
  volume={55 2},
  pages={235-46}
}
A fraction of the 28S ribosomal genes in certain insect species is interrupted by the insertion elements R1 and R2. These two elements from the silkworm Bombyx mori (R1Bm and R2Bm) are retrotransposons capable of transposing in a highly sequence-specific manner. We report here the functional expression in E. coli of the entire single open reading frame of R2Bm and show that it encodes a double-stranded endo-nuclease (integrase) that can specifically cleave the 28S gene at the R2 insertion site… CONTINUE READING