Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme.

@article{Knecht1996FunctionalEO,
  title={Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme.},
  author={Wolfgang G. Knecht and U Bergjohann and Sigrid Gonski and Barry Kirschbaum and Monika L{\"o}ffler},
  journal={European journal of biochemistry},
  year={1996},
  volume={240 1},
  pages={292-301}
}
Human mitochondrial dihydroorotate dehydrogenase (the fourth enzyme of pyrimidine de novo synthesis) has been overproduced by means of a recombinant baculovirus that contained the human cDNA fragment for this protein. After virus infection and protein expression in Trichoplusia ni cells (BTI-Tn-5B1-4), the subcellular distribution of the recombinant dihydroorotate dehydrogenase was determined by two distinct enzyme-activity assays and by Western blot analysis with anti-(dihydroorotate… CONTINUE READING

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