Functional expression of Escherichia coli endonuclease IV in apurinic endonuclease-deficient yeast.

@article{Ramotar1996FunctionalEO,
  title={Functional expression of Escherichia coli endonuclease IV in apurinic endonuclease-deficient yeast.},
  author={Dindial Ramotar and Bruce Demple},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 13},
  pages={7368-74}
}
Saccharomyces cerevisiae Apn1 and Escherichia coli endonuclease IV are homologous enzymes that initiate the repair of abasic (AP) sites or oxidative DNA strand breaks. Yeast lacking Apn1 (apn1-) are hypersensitive to simple alkylating agents (which produce many AP sites) and to oxidants and display an elevated spontaneous mutation rate due to endogenous damages. We explored whether the prokaryotic repair enzyme could substitute for its yeast counterpart. Plasmid constructs were generated that… CONTINUE READING