Functional expression of Candida antarctica lipase B in Eschericha coli.

@article{Blank2006FunctionalEO,
  title={Functional expression of Candida antarctica lipase B in Eschericha coli.},
  author={Kerstin Blank and Julia Morfill and Hermann Gumpp and Hermann Eduard Gaub},
  journal={Journal of biotechnology},
  year={2006},
  volume={125 4},
  pages={474-83}
}
Candida antarctica lipase B (CalB) is an important catalyst in bio-organic synthesis. To optimize its performance, either the reaction medium is changed or the lipase itself is modified. In the latter case, mutants are generated in Eschericha coli and subsequently expressed in fungal hosts for their characterization. Here we present the functional expression of CalB in the periplasm of E. coli. By step-wise deletion of the CalB signal and propeptide we were able to express and purify two… CONTINUE READING