Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells.

@article{Meyer1994FunctionalEA,
  title={Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells.},
  author={Tim Meyer and Peter van Endert and S Uebel and B Ehring and Robert Tamp{\'e}},
  journal={FEBS letters},
  year={1994},
  volume={351 3},
  pages={443-7}
}
Using the baculovirus expression system the gene products of human tap1 and tap2 were over-expressed as wild-type as well as oligohistidine fusion proteins in Spodoptera frugiperda (Sf9) insect cells. Both gene products were co-expressed within the same cells and were found enriched in microsomal membranes. Immunoprecipitation and immobilized metal affinity chromatography revealed complex formation between TAP1 and TAP2. The expressed TAP complex was shown to be functional by peptide… CONTINUE READING

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Antibodies, Cold Spring Harbour Laboratory

  • E. Harlow, D. Lane
  • 1988
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