Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae

Abstract

Cytokinin dehydrogenase (CKX) is responsible for regulating the endogenous cytokinin content by oxidative removal of the side chain and seven distinct genes, AtCKX1 to AtCKX7, code for the enzyme in Arabidopsis thaliana. The recombinant enzyme AtCKX2 was produced in Saccharomyces cerevisiae after expressing the corresponding gene from a plasmid (pDR197) or… (More)
DOI: 10.1007/s10535-007-0141-6

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@article{Frbortov2007FunctionalEA, title={Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae}, author={Jitka Fr{\'e}bortov{\'a} and Petr Galuszka and Theresa Werner and Thomas Schm{\"{u}lling and Ivo Fr{\'e}bort}, journal={Biologia Plantarum}, year={2007}, volume={51}, pages={673-682} }