Functional evidence for active site location of tetrameric thymidylate synthase X at the interphase of three monomers.

@article{Leduc2004FunctionalEF,
  title={Functional evidence for active site location of tetrameric thymidylate synthase X at the interphase of three monomers.},
  author={Damien Leduc and S{\'e}bastien Graziani and G{\'e}rard Lipowski and Christophe Marchand and Pierre Le Mar{\'e}chal and Ursula Liebl and Hannu Myllykallio},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 19},
  pages={7252-7}
}
Little is known about the catalytic mechanism of the recently discovered ThyX family of flavin-dependent thymidylate synthases that are required for thymidylate (deoxythymidine 5'-monophosphate) synthesis in a large number of microbial species. Using a combination of site-directed mutagenesis and biochemical measurements, we have identified several residues of the Helicobacter pylori ThyX protein with crucial roles in ThyX catalysis. By providing functional evidence that the active site(s) of… CONTINUE READING

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