Functional domains of the 50S subunit mature late in the assembly process

  title={Functional domains of the 50S subunit mature late in the assembly process},
  author={Ahmad Jomaa and Nikhil Jain and Joseph H Davis and James R Williamson and Robert A Britton and Joaquin Ortega},
  booktitle={Nucleic acids research},
Despite the identification of many factors that facilitate ribosome assembly, the molecular mechanisms by which they drive ribosome biogenesis are poorly understood. Here, we analyze the late stages of assembly of the 50S subunit using Bacillus subtilis cells depleted of RbgA, a highly conserved GTPase. We found that RbgA-depleted cells accumulate late assembly intermediates bearing sub-stoichiometric quantities of ribosomal proteins L16, L27, L28, L33a, L35 and L36. Using a novel pulse… CONTINUE READING
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