Functional domains of an ATP-dependent DNA ligase.

@article{Doherty1999FunctionalDO,
  title={Functional domains of an ATP-dependent DNA ligase.},
  author={Aidan J. Doherty and Dale B. Wigley},
  journal={Journal of molecular biology},
  year={1999},
  volume={285 1},
  pages={
          63-71
        }
}
The crystal structure of an ATP-dependent DNA ligase from bacteriophage T7 revealed that the protein comprised two structural domains. In order to investigate the biochemical activities of these domains, we have overexpressed them separately and purified them to homogeneity. The larger N-terminal domain retains adenylation and ligase activities, though both at a reduced level. The adenylation activity of the large domain is stimulated by the presence of the smaller domain, suggesting that a… CONTINUE READING

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