Functional diversity of cytochrome P450s of the white-rot fungus Phanerochaete chrysosporium.

Abstract

The functional diversity of cytochrome P450s (P450s) of the white-rot basidiomycete, Phanerochaete chrysosporium, was studied. A series of compounds known to be P450 substrates of other organisms were utilized for metabolic studies of P. chrysosporium. Metabolic conversions of benzoic acid, camphor, 1,8-cineol, cinnamic acid, p-coumaric acid, coumarin, cumene, 1,12-dodecanediol, 1-dodecanol, 4-ethoxybenzoic acid, and 7-ethoxycoumarin were observed with P. chrysosporium for the first time. 1-Dodecanol was hydroxylated at seven different positions to form 1,12-, 1,11-, 1,10-, 1,9-, 1,8-, 1,7-, and 1,6-dodecandiols. The effect of piperonyl butoxide, a P450 inhibitor, on the fungal conversion of 1-dodecanol was also investigated, indicating that hydroxylation reactions of 1-dodecanol were inhibited by piperonyl butoxide in a concentration-dependent manner. With 11 substrates, 23 hydroxylation reactions and 2 deethylation reactions were determined and 6 products were new with the position of hydroxyl group incorporated. In conclusion, fungal P450s were shown to have diverse and unique functions.

0100200300'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

546 Citations

Semantic Scholar estimates that this publication has 546 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Matsuzaki2004FunctionalDO, title={Functional diversity of cytochrome P450s of the white-rot fungus Phanerochaete chrysosporium.}, author={Fumiko Matsuzaki and Hiroyuki Wariishi}, journal={Biochemical and biophysical research communications}, year={2004}, volume={324 1}, pages={387-93} }