Functional dissection of the human Bcl2 protein: sequence requirements for inhibition of apoptosis.

@article{Hunter1996FunctionalDO,
  title={Functional dissection of the human Bcl2 protein: sequence requirements for inhibition of apoptosis.},
  author={John J. Hunter and Bruce Bond and Tristram G. Parslow},
  journal={Molecular and cellular biology},
  year={1996},
  volume={16 3},
  pages={
          877-83
        }
}
Overexpression of the cytoplasmic oncoprotein Bcl2 blocks programmed cell death (apoptosis) in many cellular systems. To map the sequences in Bcl2 that are necessary for its activity, we created a library of deletion-scanning mutants of this 239-amino-acid protein and tested their abilities to block staurosporine-induced fibroblast apoptosis, using a novel transient-transfection assay. Phenotypes of informative mutants were then confirmed by assaying for inhibition of steroid-induced apoptosis… CONTINUE READING

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